Journal: Frontiers in Endocrinology

Article Title: Identification and Expression Analysis of G Protein-Coupled Receptors in the Miridae Insect Apolygus lucorum

doi: 10.3389/fendo.2021.773669

Figure Lengend Snippet: Neuropeptide and protein hormone receptors and purine GPCRs in A. lucorum .

Article Snippet: A89 , KAF6200805.1 , Orphan , na , na , 6 , (IPR000276) GPCR, rhodopsin-like , 1.02E−12 , Melatonin receptor type 1A , Gallus gallus.

Techniques:

Journal: Cells

Article Title: Activation of MT1/MT2 to Protect Testes and Leydig Cells against Cisplatin-Induced Oxidative Stress through the SIRT1/Nrf2 Signaling Pathway

doi: 10.3390/cells11101690

Figure Lengend Snippet: Melatonin could attenuate the downregulation of the MT1, MT2, and SIRT1/Nrf2 signaling pathways by cisplatin in Leydig cells. ( A ) Determination of MT1 and MT2 protein levels was performed by Western blotting in Leydig cells treated with different concentrations of melatonin for 24 h. The blots were quantified using Image J software. ( B ) MT1 immunofluorescence in Leydig cells treated with 1 μM of melatonin. MT1 was tagged with green fluorescence. The nucleus was labeled with DAPI (scale bar = 20 μm). ( C ) MT2 immunofluorescence in Leydig cells treated with 1 μM of melatonin. MT1 was tagged with red fluorescence. The nucleus was labeled with DAPI (scale bar = 20 μm). ( D ) Determination of Nrf2, HO-1, and NQO1 protein levels was performed by Western blotting in cisplatin-induced Leydig cells pretreated with different concentrations of melatonin or ramelteon. The blots were quantified using Image J software. ( E ) Determination of the SIRT1, SOD1, and SOD2 protein levels was performed by Western blotting in cisplatin-induced Leydig cells pretreated with different concentrations of melatonin or ramelteon. The blots were quantified using Image J software. CP, cisplatin. Mel, melatonin. Data are presented as the mean ± SEM ( N = 4 per group). * p < 0.05 or ** p < 0.01 compared with controls. # p < 0.05 compared with CP groups. ∆ p < 0.05 compared with CP groups.

Article Snippet: Melatonin (Cat#: 73-31-4) and cisplatin (Cat#: 15663-27-1) were purchased from Sigma-Aldrich (St Louis, MO, USA), whereas the melatonin-receptor antagonist Luzindole (Cat#: 117946-91-5) and melatonin-receptor agonist Ramelteon (Cat#: 196597-26-9) were purchased from MedChemExpress, LLC (Medchemexpress, Shanghai, China).

Techniques: Protein-Protein interactions, Western Blot, Software, Immunofluorescence, Fluorescence, Labeling

Journal: International journal of molecular sciences

Article Title: Melatonin Receptor Expression in Primary Uveal Melanoma.

doi: 10.3390/ijms25168711

Figure Lengend Snippet: Figure 2. MTNR1A and MTNR1B receptors in the nucleus vs. cytoplasm. (A) The mean optical density (OD) for MTNR1A was significantly higher in the cytoplasm compared to the nucleus (p < 0.0001, n = 46); (B) The mean optical density (OD) for MTNR1B was significantly higher in the cytoplasm compared to the nucleus (p < 0.0001, n = 45).

Article Snippet: Formalin-fixed paraffin-embedded tissue from the enucleated eyes were sectioned and stained on a BOND III IHC stainer by biomedical analysts using mouse monoclonal antibodies to detect melatonin receptor 1A (NBP3-03633, Novus Biologicals, Centennial, CO, USA) and melatonin receptor 1B (NLS932, Novus Biologicals, Centennial, CO, USA).

Techniques:

Journal: International journal of molecular sciences

Article Title: Melatonin Receptor Expression in Primary Uveal Melanoma.

doi: 10.3390/ijms25168711

Figure Lengend Snippet: Figure 3. Kaplan–Meier survival curves and scatter plots for MTNR1B expression. (A) Survival curve comparing the incidence of UM-related death in those with MTNR1B OD levels in the nucleus below or equal to and above the median value (p = 0.98), n = 45. (B) Survival curve comparing incidence of UM-related death in those with MTNR1B OD levels in the cytoplasm below or equal to and above the median value (p = 0.34), n = 45. (C) Scatter plot comparing MTNR1B RNA expression in patient tumors with disomy 3 or monosomy 3. (D) Scatter plot comparing MTNR1B RNA expression in patients with tumors of an epithelioid cell type compared to other cell types (spindle or mixed). (E) Scatter plot comparing MTNR1B RNA expression in patients with the BAP1 wild type or BAP1 mutation. (F) Scatter plot comparing MTNR1B OD levels in the nucleus of tumor cells for patients who did or did not die due to uveal melanoma. (G) Scatter plot comparing MTNR1B OD levels in the cytoplasm of tumor cells for patients who did or did not die due to uveal melanoma. Colored fields on the Kaplan–Meier curves indicate 95% confidence intervals. ns = not significant. TCGA cohort, St Erik cohort.

Article Snippet: Formalin-fixed paraffin-embedded tissue from the enucleated eyes were sectioned and stained on a BOND III IHC stainer by biomedical analysts using mouse monoclonal antibodies to detect melatonin receptor 1A (NBP3-03633, Novus Biologicals, Centennial, CO, USA) and melatonin receptor 1B (NLS932, Novus Biologicals, Centennial, CO, USA).

Techniques: Expressing, RNA Expression, Mutagenesis